Thermo Fisher Scientific Serpina12 Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MLKPSFSPRN YKALSEVQGW KQRMAAKELA RQNMDLGFKL LKKLAFYNPG RNIFLSPLSI STAFSMLCLG AQDSTLDEIK QGFNFRKMPE KDLHEGFHYI IHELTQKTQD LKLSIGNTLF IDQRLQPQRK FLEDAKNFYS AETILTNFQN LEMAQKQIND FISQKTHGKI NNLIENIDPG TVMLLANYIF FRARWKHEFD PNVTKEEDFF LEKNSSVKVP MMFRSGIYQV GYDDKLSCTI LEIPYQKNIT AIFILPDEGK LKHLEKGLQV DTFSRWKTLL SRRVVDVSVP RLHMTGTFDL KKTLSYIGVS KIFEEHGDLT KIAPHRSLKV GEAVHKAELK MDERGTEGAA GTGAQTLPME TPLVVKIDKP YLLLIYSEKI PSVLFLGKIV NPIGK.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Vaspin. Anti-Human Vaspin-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hVaspin by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Vaspin (500-P256) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hVaspin.

Western Blot:
To detect hVaspin by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant hVaspin is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P256BT-1MG will be provided as 2 × 500 µg.

Target Information

Vaspin is a newly described adipocytokine expressed predominantly in visceral white adipose tissues. Structure analysis of vaspin predicts the presence of three beta-sheets, nine alpha-helices, and one central loop, which are distinctive structural features of Serpin family members.

The serpins are irreversible (“suicidal”) serine-protease inhibitors, characterized by having more than 30% sequence homology with alpha1-antitrypsin and a conserved tertiary structure containing an exposed reactive center loop acting as a pseudo-substrate for the target proteinase.

Members of this family play an important role in biological processes such as:

• Blood coagulation
• Fibrinolysis
• Complement activation
• Angiogenesis
• Inflammation
• Tumor suppression

In humans, serpins represent approximately 2% of total serum proteins, of which 70% is alpha1-antitrypsin. Vaspin exhibits 40.2% sequence identity with alpha1-antitrypsin, though its protease inhibitory activity is still unknown.

Vaspin mRNA expression in visceral fat correlates positively with BMI and body fat percentage. Administration of vaspin to obese mice improved glucose tolerance and insulin sensitivity, normalizing blood glucose levels and reversing altered expression of diabetes-related adipocytokines such as leptin, adiponectin, resistin, and TNF-alpha.

These findings suggest a potential clinical use for Vaspin in ameliorating metabolic syndrome-related abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.