Thermo Fisher Scientific Cytohesin 1/2 Monoclonal Antibody (6G11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

-

Immunocytochemistry (ICC/IF)

1:100

-

Immunoprecipitation (IP)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Not Applicable

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

6G11

Immunogen

Purified, His-tagged cytohesin-1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

PEG precipitation

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2089016

Product Specific Information

MA1-062 detects recombinant human and mouse cytohesin-1 and 2 (ARNO).

MA1-062 has been successfully used in Western blot, IF and immunoprecipitation procedures. By Western blot, this antibody detects an ~48 kDa band representing both cytohesin-1 and cytohesin-2. In immunoprecipitation procedures MA1-062 only precipitates cytohesin-2.

The MA1-062 immunogen is purified, His-tagged cytohesin-1.

Target Information

The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. This gene is highly expressed in natural killer and peripheral T cells, and regulates the adhesiveness of integrins at the plasma membrane of lymphocytes. The encoded protein is 83% homologous to that of CYTH2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.