Thermo Fisher Scientific Phospho-4EBP1 (Thr36, Thr45) Monoclonal Antibody (V3NTY24), eFluor 660, eBioscience
인간 및 마우스 4EBP1의 Thr36/Thr45 인산화형을 인식하는 단클론 항체로, eFluor 660 형광체 결합. 세포내 염색 및 유세포 분석에 적합하며, mTOR 관련 신호전달 연구에 활용 가능. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific Phospho-4EBP1 (Thr36, Thr45) Monoclonal Antibody (V3NTY24), eFluor 660
Applications
- Flow Cytometry (Intracellular staining)
Tested Dilution: 5 µL (0.06 µg)/test
Publications: View 2 publications
Miscellaneous PubMed
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 660 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | V3NTY24 |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic), Wet ice (international) |
| RRID | AB_2574318 |
Product Specific Information
The V3NTY24 monoclonal antibody recognizes human and mouse eukaryotic translation initiation factor eIF4E-binding protein 1 (4E-BP1) when phosphorylated at threonine 37 and/or threonine 46.
4E-BP1 binds to eIF4E and represses cap-dependent translation in its non-phosphorylated form. Phosphorylation at multiple sites disrupts this interaction, enabling translation.
Kinases such as FRAP/mTOR phosphorylate Thr37 and Thr46, while ATM phosphorylates Ser111. PI3 kinase inhibitors LY294002 and wortmannin inhibit phosphorylation at Thr37/Thr46.
Applications Reported:
- Intracellular staining followed by flow cytometric analysis.
Applications Tested:
- Pre-titrated and validated for intracellular staining and flow cytometry of stimulated normal human peripheral blood cells.
- Recommended usage: 5 µL (0.06 µg) per test in 100 µL final volume (10⁵–10⁸ cells/test).
Protocols:
- Protocol A: Two-step protocol for intracellular (cytoplasmic) proteins.
- Protocol C: Two-step protocol with fixation/methanol for phospho-specific discrimination.
- Protocol B: One-step nuclear protein staining not recommended.
Detailed protocols available in BestProtocols®.
Fluorochrome Information:
- eFluor® 660 replaces Alexa Fluor® 647.
- Emission: 659 nm; Excitation: 633–647 nm (Red laser).
- Ensure instrument compatibility.
- Filtered post-manufacturing (0.2 µm).
Target Information
This gene encodes a translation repressor protein that interacts with eukaryotic translation initiation factor 4E (eIF4E), inhibiting complex assembly and repressing translation.
Phosphorylation in response to stimuli such as UV irradiation or insulin signaling dissociates it from eIF4E, activating mRNA translation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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