Vazyme T7 High Yield RNA Transcription Kit TR101

Product Introduction

The T7 High Yield Transcription Kit is an optimized system for high yield in vitro transcription of RNA from DNA templates containing T7 RNA Polymerase promoter. The kit contains T7 RNA Polymerase which can synthesize RNA quickly and easily from the downstream of the T7 promoter, and obtains a large amount of RNA. Modified nucleotide can be added to the system to generate biotin or dye-labeled RNA.
This kit can yield 150 - 200 μg of RNA with a template input of 0.5 μg. The RNA yield can be widely used in many downstream applications such as RNA structure and function studies, RNase protection, probe hybridization, RNAi, microinjection and in vitro translation.

Product Advantages

Optimized reaction system;
Each reaction produces up to 150-200 μg of RNA product

Components

Storage

Store at -30 ~ -15℃ for up to 18 months, transport at ≤0℃.

Citation

Lyu, Jialan, et al. "The conserved microRNA miR-210 regulates lipid metabolism and photoreceptor maintenance in the Drosophila retina." Cell Death & Differentiation 28.2 (2021): 764-779.

Sui, Baokun, et al. "A novel antiviral lncRNA, EDAL, shields a T309 O-GlcNAcylation site to promote EZH2 lysosomal degradation." Genome biology 21.1 (2020): 1-36.

Chen, Peng, et al. "A Cas12a ortholog with stringent PAM recognition followed by low off-target editing rates for genome editing." Genome biology 21.1 (2020): 1-13.

Feng, Yue, et al. "METTL3/METTL14 Transactivation and m6A-Dependent TGF-β1 Translation in Activated Kupffer Cells." Cellular and Molecular Gastroenterology and Hepatology 12.3 (2021): 839-856.

Zhao, Jing, et al. "Proteomic analyses of whitefly-begomovirus interactions reveal the inhibitory role of tumorous imaginal discs in viral retention." Frontiers in immunology (2020): 1596.

Application Notes Abstract

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