Thermo Fisher Scientific GFP Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

PA1-9533 detects Green Fluorescence Protein in N/A samples.
It has been successfully used in Western blot, ELISA, and immunocytochemistry procedures.
By Western blot, PA1-9533 detects a 32 kDa protein representing Green Fluorescence Protein.
The immunogen is a purified recombinant green fluorescence protein.

Target Information

The jellyfish Aequorea victoria contains Green Fluorescent Protein (GFP Tag) that emits light in the bioluminescence reaction of the animal.
GFP is widely used as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms.
As a fusion tag, GFP helps localize proteins, study their movement, and analyze subcellular dynamics.
GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment.
It absorbs blue light (max 395 nm) and emits green light (peak 508 nm) without additional proteins or cofactors.
GFP fluorescence is stable, species-independent, and suitable for various applications including monitoring gene expression, protein localization, protein-protein interactions (yeast two-hybrid system), and fluorescence energy transfer (FRET) experiments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.