Thermo Fisher Scientific 6x-His Tag Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

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Immunocytochemistry (ICC/IF)

1:400-1:2,000

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Flow Cytometry (Flow)

-

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ELISA (ELISA)

1:1,000-1:5,000

-

GST Pull Down (GPD)

-

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Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

His tagged synthetic peptide.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1069887

Product Specific Information

PA1-9531 has been successfully used in Western blot, ELISA and immunocytochemistry procedures. By Western blot, PA1-9531 detects a His-Tag fusion protein protein representing His Tag.

PA1-9531 immunogen is a His tagged synthetic peptide.

Target Information

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.