Thermo Fisher Scientific CD53 Chimeric Recombinant Rabbit Monoclonal Antibody (161-2 (53/2))
인간 CD53 항원을 특이적으로 인식하는 재조합 토끼 단클론 항체. ICC/IF, Flow Cytometry 등 다양한 응용 가능. HEK293 세포 발현, Protein A 정제, 1 mg/mL 농도. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | Assay-dependent |
| Functional Assay (Functional) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 161-2 (53/2) |
| Immunogen | This antibody was raised by immunizing mice with stimulated human leukocytes. Target family: CD53 (UniProt ID: P19397-1), antigen range: 1–219. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074714 |
Product Specific Information
Specificity: This antibody recognizes human CD53. It does not react with baboon and horse antigen.
CD53 is a member of the tetraspanin family and is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins.
Target Information
CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage.
It forms complexes with leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4, and tetraspanins CD37, CD81, CD82, modulating signaling processes.
CD53 contributes to tumor cell radioresistance and exhibits anti-apoptotic effects upon activation.
In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and strongly expressed upon macrophage exposure to bacterial lipopolysaccharide.
Stimulation of neutrophils results in down-regulation of CD53 expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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