Thermo Fisher Scientific Phospho-WWTR1 (Ser66) Polyclonal Antibody
Phospho-WWTR1 (Ser66) 특이적 항체로, 인산화된 TAZ 단백질 검출에 사용됩니다. Western blot 및 면역세포화학에 적합하며, 인간, 마우스, 랫트 시료에 반응합니다. PBS 기반 완충액에 보관되며 -20°C에서 안정적으로 저장 가능합니다.
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- PA5105067
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- pk
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human WWTR1 (Accession Q9GZV5), corresponding to amino acid residues around phosphorylated Ser66 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816540 |
Product Specific Information
This antibody detects endogenous levels of TAZ only when phosphorylated at Ser66.
Target Information
WW domain-containing transcription regulator protein 1 (WWTR1) is a transcriptional coactivator that functions as a downstream regulatory target in the Hippo signaling pathway. This pathway plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis.
The core of this pathway includes a kinase cascade where STK3/MST2 and STK4/MST1, in complex with SAV1, phosphorylate and activate LATS1/2 with MOB1. MOB1 subsequently phosphorylates and inactivates YAP1 and WWTR1/TAZ.
WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation, regulates nuclear accumulation of SMADs, and couples them to transcriptional machinery such as the mediator complex. It also contributes to embryonic stem-cell self-renewal, cell proliferation, and epithelial-mesenchymal transition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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