Thermo Fisher Scientific NSE Monoclonal Antibody (5E2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:2,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:300

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ELISA (ELISA)

1:100-1:2,000

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Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Published species

Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

5E2

Immunogen

Purified full length native human NSE protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

3.1 mg/mL

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_568652

Product Specific Information

Antigen retrieval is not required but may optimize staining of paraffin tissue sections.

Suggested positive control: antigen standard for ENO2 (transient overexpression lysate).

Target Information

Neuron specific enolase (NSE, ENO1, ENO2, ENO3) is an enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and the reverse reaction in gluconeogenesis. NSE has a high stability in biological fluids and can easily diffuse to the extracellular medium and cerebrospinal fluid (CSF) when neuronal membranes are injured.NSE is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The alpha-subunit is expressed in most tissues, the beta-subunit only in muscle, and the gamma-subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Co-expression of NSE and chromogranin A is common in neuroendocrine neoplasms. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to NSE protein are useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury. NSE is used clinically as a sensitive and useful marker of neuronal damage in several neurological disorders including stroke, hypoxic brain damage, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis. Further, NSE is found in elevated concentrations in plasma and certain neoplasias that include pediatric neuroblastoma and small cell lung cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.