Thermo Fisher Scientific Troponin I Monoclonal Antibody (12F10)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Suggested positive control: antigen standard for TNNT1 (transient overexpression lysate).

Target Information

Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles.
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), forms the troponin complex of thin filaments in striated muscle.
TnI acts as the inhibitory subunit, blocking actin–myosin interactions and mediating muscle relaxation.

The TnI subfamily includes three genes:

• TnI-skeletal-fast-twitch
• TnI-skeletal-slow-twitch
• TnI-cardiac

TnI-fast and TnI-slow are expressed in fast- and slow-twitch skeletal muscle fibers, respectively, while TnI-cardiac is expressed exclusively in cardiac muscle tissue.
This gene encodes the Troponin-I-skeletal-slow-twitch protein, expressed in cardiac and skeletal muscle during early development but restricted to slow-twitch skeletal fibers in adults.
The protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin–myosin complexes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.