Thermo Fisher Scientific Troponin I Monoclonal Antibody (12F10)
Troponin I 단백질을 인식하는 Mouse monoclonal antibody (clone 12F10). Western blot 및 ELISA에 사용 가능하며, 액상 형태로 제공. 4°C 보관, 동결 금지. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:2,000 |
| ELISA | 1:100–1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 12F10 |
| Immunogen | Full length native protein (purified, Human) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 4 mg/mL |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Wet ice |
| RRID | AB_2272089 |
Product Specific Information
- Suggested positive control: antigen standard for TNNT1 (transient overexpression lysate).
Target Information
Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles.
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), forms the troponin complex of thin filaments in striated muscle.
TnI acts as the inhibitory subunit, blocking actin–myosin interactions and mediating muscle relaxation.
The TnI subfamily includes three genes:
- TnI-skeletal-fast-twitch
- TnI-skeletal-slow-twitch
- TnI-cardiac
TnI-fast and TnI-slow are expressed in fast- and slow-twitch skeletal muscle fibers, respectively, while TnI-cardiac is expressed exclusively in cardiac muscle tissue.
This gene encodes the Troponin-I-skeletal-slow-twitch protein, expressed in cardiac and skeletal muscle during early development but restricted to slow-twitch skeletal fibers in adults.
The protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin–myosin complexes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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