Thermo Fisher Scientific Phospho-B-Raf (Thr401) Recombinant Rabbit Monoclonal Antibody (JJ08-72)

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2000
• Publications: View 1 publication

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:500

Product Specifications

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.

Advantages:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity due to larger rabbit immune repertoire

Target Information

BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutation in human cancer and plays a role in postsynaptic responses of hippocampal neurons. This protein regulates the MAP kinase/ERKs signaling pathway, influencing cell division, differentiation, and secretion.

Mutations in B-Raf are associated with various cancers, including:

• Non-Hodgkin lymphoma
• Colorectal cancer
• Malignant melanoma
• Thyroid carcinoma
• Non-small cell lung carcinoma
• Adenocarcinoma of lung

A pseudogene located on chromosome X has been identified for B-Raf.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.