Thermo Fisher Scientific A-Raf Recombinant Rabbit Monoclonal Antibody (JJ08-68)
Recombinant rabbit monoclonal antibody targeting human A-Raf protein. Validated for WB, IHC, ICC/IF, and Flow Cytometry. Offers high specificity, sensitivity, and lot-to-lot consistency. Supplied in liquid form, 1 mg/mL concentration, Protein A purified.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JJ08-68 |
| Immunogen | Recombinant protein within Human A-RAF aa 146–310 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4 °C (short term). For long term, store at −20 °C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809706 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin–free formulations
- Broad immunoreactivity due to large rabbit immune repertoire
Target Information
Several serine/threonine protein kinases act as intermediates in signal transduction pathways, including ERK/MAP kinases, ribosomal S6 kinase (Rsk), and Raf‑1. Raf‑1 is a cytoplasmic protein with intrinsic serine/threonine activity and is broadly expressed in most cell lines. It is the cellular homolog of v‑Raf, the transforming gene product of murine sarcoma virus 3611. Unregulated v‑Raf kinase activity is associated with transformation and mitogenesis, while Raf‑1 activity is normally suppressed by its N‑terminal regulatory domain.
A‑Raf, another member of the Raf family, shares substantial homology with Raf‑1 within the kinase domain but less elsewhere. A‑Raf expression is highest in urogenital tissues and kidney, and lowest in brain tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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