
Thermo Fisher Scientific Cytokeratin 2e Recombinant Rabbit Monoclonal Antibody (JJ201-8)
Recombinant rabbit monoclonal antibody for Cytokeratin 2e detection. Validated for WB and IHC (P). Offers high specificity, sensitivity, and lot-to-lot consistency. Supplied as liquid, 1 mg/mL, Protein A purified, stored at 4°C or -20°C.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JJ201-8 |
| Immunogen | Recombinant protein within Human Cytokeratin 2e aa 273–442 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809701 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and expressed in HEK293 cells. Individual clones are screened to select optimal candidates for production.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Cytokeratins are intermediate filament proteins with high biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range from 40–68 kDa in molecular weight and pI 4.9–7.8. Expression patterns of cytokeratins are characteristic of epithelial type and differentiation stage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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