Thermo Fisher Scientific MFN2 Monoclonal Antibody (N153/5), FITC
MFN2 단백질을 인식하는 FITC 결합 단클론 항체로, Western blot, IHC, ICC/IF 등 다양한 응용에 적합. 인간, 생쥐, 랫드 반응성. 단백질 G 정제, 1 mg/mL 농도, 4°C 암소 보관. 미토콘드리아 융합 및 신경 질환 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N153/5 |
| Immunogen | Fusion protein amino acids 370–600 (cytoplasmic N-terminus) of mouse Mitofusin-2 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932124 |
Available Formats
- Unconjugated (MA5-27647)
- APC (MA5-45669)
- PE (MA5-45672)
- PerCP (MA5-45671)
- Custom conjugation available upon request
Product Specific Information
- Rat: 97% identity (226/231 amino acids identical)
- Human: 92% identity (214/231 amino acids identical)
- ~55% identity with Mitofusin-1
- 1 µg/mL of MA5-45670 detects Mitofusin-2 in 20 µg of mouse cardiac mitochondrial lysate by colorimetric immunoblot using Goat anti-mouse IgG:HRP as secondary antibody
- Detects approximately 90 kDa
- No cross-reactivity against Mitofusin-1
- Formerly sold as clone S153-5
Target Information
This gene encodes a mitochondrial membrane protein involved in mitochondrial fusion and maintenance of the mitochondrial network. It regulates vascular smooth muscle cell proliferation and may be implicated in obesity-related pathophysiology. Mutations cause Charcot-Marie-Tooth disease type 2A2 and hereditary motor and sensory neuropathy VI, both affecting the peripheral nervous system. Defects have also been linked to early-onset stroke. Two transcript variants encoding the same protein are known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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