Thermo Fisher Scientific Wilms Tumor 1 (WT1) Recombinant Rabbit Monoclonal Antibody (WT1, 1434R)
Human WT1 단백질을 인식하는 Recombinant Rabbit Monoclonal 항체로, IHC(P)에서 1–2 µg/mL 농도로 사용 가능. Protein A/G로 정제된 액상 형태이며 PBS buffer(pH 7.4, 0.05% BSA, 0.05% sodium azide)로 보존. 4°C 보관, 연구용 전용.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
- Publications: [References not listed]
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | WT1, 1434R |
| Immunogen | Recombinant human full-length WT1 protein (UniProt ID: P19544-1, amino acids 1–449) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA-fixed): Incubate antibody for 30 minutes at room temperature.
- For formalin-fixed tissues: Heat sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes before staining.
Target Information
The Wilms Tumor 1 (WT1) gene encodes one of the WPP domain-interacting proteins required for RanGAP nuclear envelope association in root tip cells.
Ran GTPase is essential for nucleocytoplasmic transport, spindle formation, and postmitotic nuclear envelope reassembly.
WT1 and WIT2 are CC-TMD family proteins distinct from WIP, both necessary for RanGAP nuclear envelope association in plant and metazoan cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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