Thermo Fisher Scientific Wilms Tumor 1 (WT1) Recombinant Rabbit Monoclonal Antibody (WT1, 1434R)
인간 WT1 단백질을 인식하는 재조합 토끼 단클론 항체. IHC(P) 실험에 적합하며, 고품질 Protein A/G 정제 및 안정적 PBS 버퍼로 제공. 포르말린 고정 조직 염색 시 항원 회복 절차 포함. 연구용으로만 사용 가능.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | WT1, 1434R |
| Immunogen | Recombinant human full-length WT1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
The Wilms Tumor 1 (WT1) gene encodes one of the WPP domain-interacting proteins required for RanGAP nuclear envelope association in root tip cells.
Ran GTPase plays essential roles in nucleocytoplasmic transport, spindle formation, and nuclear envelope reassembly.
WT1 and WIT2 are CC-TMD proteins necessary for RanGAP nuclear envelope association in root tip cells, structurally distinct from WIP family proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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