Thermo Fisher Scientific Wilms Tumor 1 (WT1) Recombinant Rabbit Monoclonal Antibody (WT1, 3477R)

Applications and Tested Dilution

• Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

The Wilms Tumor 1 (WT1) gene encodes one of the WPP domain-interacting proteins required for RanGAP nuclear envelope association in root tip cells. Ran GTPase plays essential roles in nucleocytoplasmic transport, spindle formation, and postmitotic nuclear envelope reassembly. The cytoplasmic RanGAP is critical for establishing a functional RanGTP/RanGDP gradient across the nuclear envelope and is associated with the outer surface of the nuclear envelope in metazoan and higher plant cells.
Arabidopsis thaliana RanGAP association with the root tip nuclear envelope requires a family of plant-specific nucleoporins combining coiled-coil and transmembrane domains (CC-TMD) and WPP domain-interacting proteins (WIPs). WT1 and WIT2 form a second family of CC-TMD proteins that are structurally similar yet distinct from the WIP family, both required for RanGAP nuclear envelope association in root tip cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.