Thermo Fisher Scientific Wilms Tumor 1 (WT1) Recombinant Mouse Monoclonal Antibody (rWT1, 857)
Thermo Fisher Scientific의 WT1 단백질에 특이적인 재조합 마우스 단클론 항체입니다. IHC(P) 및 Peptide Array에 사용 가능하며, 인간, 마우스, 랫트에 반응합니다. Protein A/G로 정제된 액상 형태로, 보존제 없이 PBS(pH 7.4)에 보관됩니다.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
Peptide Array (Array)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rWT1, 857 |
| Immunogen | Recombinant full-length human WT1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed):
Incubate antibody for 30 minutes at room temperature (RT).
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at RT for 20 minutes.
Target Information
The Wilms Tumor 1 (WT1) gene encodes one of the WPP domain-interacting proteins required for RanGAP nuclear envelope association in root tip cells.
Ran GTPase is essential for nucleocytoplasmic transport, spindle formation, and postmitotic nuclear envelope reassembly.
WT1 and WIT2 are identified as a second family of CC-TMD proteins, structurally similar yet distinct from the WIP family, necessary for RanGAP nuclear envelope association in root tip cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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