Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), Brilliant Violet 421, eBioscience

Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), Brilliant Violet 421, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.25 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805 series
• Brilliant Violet 480–786 series
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow series
• PE, PE-Cyanine5, 5.5, 7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436–780
• Custom conjugation available

Product Specific Information

The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed by B cells during all developmental stages except terminally differentiated plasma cells. Follicular dendritic cells also express CD19. CD19 forms a multimolecular complex with CD21, CD81, MHC class II, and the BCR, mediating tyrosine phosphorylation, calcium flux, and B cell proliferation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes; ≤0.25 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best performance.
• Fluorochrome: Brilliant Violet™ 421 (BV421) emits at 423 nm, excited by 405 nm violet laser. Ensure cytometer compatibility.

When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Excitation: 407 nm
Emission: 423 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on all peripheral B cells and some myeloid leukemia cells. CD19 acts as a signal-amplifying co-receptor for the B cell receptor (BCR), regulating normal and malignant B cell proliferation. Mutations can lead to hypogammaglobulinemia, while overexpression causes B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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