Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), Brilliant Violet 421, eBioscience
마우스 CD19를 인식하는 eBio1D3 클론의 Rat IgG2a 단클론 항체로, Brilliant Violet 421 형광체가 결합되어 있습니다. Flow Cytometry용으로 검증되었으며 B세포 분석에 적합합니다. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), Brilliant Violet 421, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.25 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Brilliant Violet™ 421 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio1D3 (1D3) |
| Conjugate | Brilliant Violet™ 421 |
| Excitation / Emission Max | 406 / 423 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2929067 |
Available Formats
- Unconjugated
- Alexa Fluor 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805 series
- Brilliant Violet 480–786 series
- eFluor 450, 506, 660
- FITC
- Functional Grade
- NovaFluor Blue, Red, Violet, Yellow series
- PE, PE-Cyanine5, 5.5, 7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436–780
- Custom conjugation available
Product Specific Information
The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed by B cells during all developmental stages except terminally differentiated plasma cells. Follicular dendritic cells also express CD19. CD19 forms a multimolecular complex with CD21, CD81, MHC class II, and the BCR, mediating tyrosine phosphorylation, calcium flux, and B cell proliferation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested on mouse splenocytes; ≤0.25 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best performance.
- Fluorochrome: Brilliant Violet™ 421 (BV421) emits at 423 nm, excited by 405 nm violet laser. Ensure cytometer compatibility.
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.
Excitation: 407 nm
Emission: 423 nm
Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on all peripheral B cells and some myeloid leukemia cells. CD19 acts as a signal-amplifying co-receptor for the B cell receptor (BCR), regulating normal and malignant B cell proliferation. Mutations can lead to hypogammaglobulinemia, while overexpression causes B cell hyperactivity.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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