Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), Brilliant Violet 421, eBioscience

Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), Brilliant Violet 421, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test

Species Reactivity

Baboon, Chimpanzee, Cynomolgus monkey, Human, Rhesus monkey

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV Series (395–805 nm)
• Brilliant Violet Series (480–786 nm)
• eFluor 506
• FITC
• NovaFluor Series (Blue, Red, Violet, Yellow)
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright Series (436–780 nm)
• Request custom conjugation

Product Specific Information

The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule. CD11b, associated with integrin beta2 (CD18), is expressed on monocytes, granulocytes, activated lymphocytes, and a subset of NK cells. It serves as a receptor for intercellular adhesion molecules (CD54, CD102, CD50) and iC3b, which are essential for cell-cell and cell-matrix interactions.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated for flow cytometry using normal human peripheral blood cells. Recommended usage is 5 µL (0.25 µg) per test (100 µL final volume).

Fluorochrome Information:
Brilliant Violet™ 421 (BV421) emits at 423 nm, optimized for cytometers equipped with a violet (405 nm) laser. Ensure instrument compatibility before use.

Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Excitation: 407 nm
Emission: 423 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that forms a heterodimer with integrin beta-2 (CD18), known as Mac-1 or CR3. It is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and microglia. CD11b/CD18 acts as a receptor for ICAMs, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X, facilitating critical cell-cell and matrix interactions.

CD11b is used as a microglial marker and shows variable expression across tissues and macrophage types. Dysfunction of CD11b is associated with systemic lupus erythematosus and ITGAM-related susceptibility.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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