Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), Brilliant Violet 421, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 615, 661, 737, 805
• Brilliant Violet 480, 605, 650, 711, 786
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue/Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The 61D3 monoclonal antibody reacts with human CD14, a 53–55 kDa GPI-linked glycoprotein expressed on monocytes, interfollicular macrophages, and some dendritic cells. Complexes of LPS and LBP bind with high affinity to CD14 on monocytes.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Prediluted and tested by flow cytometric analysis of normal human peripheral blood cells.
  • Recommended use: 5 µL (0.5 µg) per test in a final volume of 100 µL.
  • Cell number range: 10⁵–10⁸ cells/test.

Brilliant Violet™ 421 (BV421) emits at 423 nm and is intended for cytometers equipped with a violet (405 nm) laser. Ensure your instrument is capable of detecting this fluorochrome.

When using multiple polymer dye–conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

• Excitation: 407 nm
• Emission: 423 nm
• Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein expressed on mature monocytes, macrophages, and neutrophils. It functions as a multifunctional lipopolysaccharide receptor and exists in both membrane-bound and soluble forms. CD14 binds LPS via LBP and plays a role in immune recognition and inflammatory responses. Soluble CD14 can distinguish structural variations in LPS and contributes to neutralization of foreign lipopolysaccharides. It is also involved in binding apoptotic cells and modulating allergic, inflammatory, and infectious processes. Alternative splicing results in multiple transcript variants encoding the same isoform.

For Research Use Only. Not for use in diagnostic procedures.

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