Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), Brilliant Violet 421, eBioscience

Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), Brilliant Violet 421, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 615, 737, 805
• Brilliant Violet 605, 650, 711
• eFluor 450, 506
• FITC
• NovaFluor Blue 610-70S, 660-120S
• NovaFluor Red 710, 725
• NovaFluor Yellow 590, 660
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

The 2H7 monoclonal antibody reacts with human CD20, a 33–36 kDa transmembrane protein expressed by developing and mature B cells but not plasma cells. CD20 may play a role in B-cell activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested on normal human peripheral blood cells at 5 µL (0.5 µg) per test
• Recommended Use: For cytometers equipped with a violet (405 nm) laser detecting emission at 423 nm

When using multiple polymer dye-conjugated antibodies (e.g., Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Excitation: 407 nm
Emission: 423 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD20 is a non-glycosylated surface phosphoprotein (33–37 kDa) expressed on mature and most malignant B cells, subsets of T lymphocytes, and follicular dendritic cells. It regulates calcium conductance, cell cycle progression, and B-cell proliferation. CD20 remains on the cell surface after antibody binding, making it a useful target for B-cell depletion therapies.
Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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