
Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), Brilliant Violet 421, eBioscience
인간 CD20 항원을 인식하는 2H7 클론 단일클론항체로, Brilliant Violet 421 형광체 결합. Flow cytometry 분석에 최적화되어 있으며, 405 nm violet laser 사용 시 423 nm에서 발광. B세포 활성 및 분화 연구용. 연구용으로만 사용 가능.
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Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), Brilliant Violet 421, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.5 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), Brilliant Violet™ 421, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2H7 |
| Conjugate | Brilliant Violet™ 421 |
| Excitation / Emission Max | 406 / 423 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925496 |
Additional Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 615, 737, 805
- Brilliant Violet 605, 650, 711
- eFluor 450, 506
- FITC
- NovaFluor Blue 610-70S, 660-120S
- NovaFluor Red 710, 725
- NovaFluor Yellow 590, 660
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation available
Product Specific Information
The 2H7 monoclonal antibody reacts with human CD20, a 33–36 kDa transmembrane protein expressed by developing and mature B cells but not plasma cells. CD20 may play a role in B-cell activation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-diluted and tested on normal human peripheral blood cells at 5 µL (0.5 µg) per test
- Recommended Use: For cytometers equipped with a violet (405 nm) laser detecting emission at 423 nm
When using multiple polymer dye-conjugated antibodies (e.g., Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.
Excitation: 407 nm
Emission: 423 nm
Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.
Target Information
CD20 is a non-glycosylated surface phosphoprotein (33–37 kDa) expressed on mature and most malignant B cells, subsets of T lymphocytes, and follicular dendritic cells. It regulates calcium conductance, cell cycle progression, and B-cell proliferation. CD20 remains on the cell surface after antibody binding, making it a useful target for B-cell depletion therapies.
Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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