Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Brilliant Violet 421, eBioscience

Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Brilliant Violet 421, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
Publications: -

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805
• Brilliant Violet 605, 650, 711
• eFluor 450, 506, 615, 660
• FITC
• Functional Grade
• NovaFluor Blue 510–690
• NovaFluor Yellow 570
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 780
• Request custom conjugation

Product Specific Information

The N418 monoclonal antibody reacts with mouse CD11c (integrin alphaX). CD11c associates with beta2 integrin to form the CD11c/CD18 heterodimer. It is expressed by dendritic cells, intestinal intraepithelial lymphocytes (IEL), and some activated T cells. CD11c/CD18 binds to CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse bone marrow cells. Recommended ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best results.

Brilliant Violet™ 421 emits at 423 nm and is suitable for cytometers with a 405 nm violet laser. Ensure instrument compatibility.

When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Excitation: 407 nm
Emission: 423 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD11, along with CD18, forms a heterodimer adhesion molecule.

• CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils.
• CD11b: Expressed by monocytes, macrophages, NK cells, some B/T cells, granulocytes.
• CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and subsets of lymphocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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