Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Brilliant Violet 421, eBioscience
Mouse CD11c 단백질을 인식하는 N418 클론 단일클론 항체로, Brilliant Violet 421 형광체 결합. Flow cytometry에 최적화되어 있으며, 0.5 µg/test로 사용 권장. 4°C 암소 보관, 비동결. 연구용으로만 사용.
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Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Brilliant Violet 421, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.5 µg/test
Publications: -
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), Brilliant Violet™ 421, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N418 |
| Conjugate | Brilliant Violet™ 421 |
| Excitation/Emission Max | 406/423 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925492 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805
- Brilliant Violet 605, 650, 711
- eFluor 450, 506, 615, 660
- FITC
- Functional Grade
- NovaFluor Blue 510–690
- NovaFluor Yellow 570
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436, 600, 645, 780
- Request custom conjugation
Product Specific Information
The N418 monoclonal antibody reacts with mouse CD11c (integrin alphaX). CD11c associates with beta2 integrin to form the CD11c/CD18 heterodimer. It is expressed by dendritic cells, intestinal intraepithelial lymphocytes (IEL), and some activated T cells. CD11c/CD18 binds to CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse bone marrow cells. Recommended ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best results.
Brilliant Violet™ 421 emits at 423 nm and is suitable for cytometers with a 405 nm violet laser. Ensure instrument compatibility.
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.
Excitation: 407 nm
Emission: 423 nm
Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
CD11, along with CD18, forms a heterodimer adhesion molecule.
- CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils.
- CD11b: Expressed by monocytes, macrophages, NK cells, some B/T cells, granulocytes.
- CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and subsets of lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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