Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), eBioscience
Mouse PD-1 단백질을 인식하는 J43 클론 단일클론 항체로, Flow Cytometry, Immunoprecipitation, Functional Assay 등에 사용 가능. 0.5 mg/mL 농도의 액상 형태이며, PBS buffer에 0.09% sodium azide 포함. 면역조절 연구 및 자가면역 질환 모델에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 71 publications |
| Immunoprecipitation (IP) | Assay-Dependent | View 1 publication |
| Neutralization (Neu) | - | View 1 publication |
| Functional Assay | - | View 3 publications |
| Inhibition Assays (IA) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | J43 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468664 |
Available Conjugate Formats
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 480 / 605 / 650 / 786
- eFluor 450 / 506
- FITC
- Functional Grade
- PE / PE-Cyanine7 / PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation
Product Specific Information
The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. PD-L1 (B7-H1) and PD-L2 (B7-DC) are known ligands of PD-1, exhibiting overlapping functions and expression patterns.
J43 inhibits the binding of mouse PD-L1-Ig and PD-L2-Ig to PD-1/BHK transfected cells. In vivo, J43 enhances contact hypersensitivity and exacerbates acute GVHD, EAE, and NOD diabetes, similar to PD-1-deficient cell transfer or antibodies against PD-L1/PD-L2.
Applications Reported: Flow cytometric analysis, immunoprecipitation, and functional assays.
For functional assays, use Functional Grade purified J43 (Product #16-9985).
Applications Tested: Flow cytometry of mouse Con-A activated spleen cells and PD-1 transfected cells at ≤0.5 µg/test (100 µL final volume). Optimize antibody titration per assay.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
PD-1 (PDCD-1) is an inhibitory receptor expressed on activated T cells. It binds PD-L1 and PD-L2 on antigen-presenting cells to suppress T-cell activation in the absence of danger signals. PD-1 deficiency results in hyperactivated immune responses and autoimmune disease. PD-1 may migrate at higher molecular weight than predicted in SDS-PAGE.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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