Thermo Fisher Scientific CD184 (CXCR4) Monoclonal Antibody (2B11), eBioscience
CD184(CXCR4) 단일클론 항체(2B11)는 사람 및 마우스 시료에서 CXCR4 단백질을 검출하는 데 사용됩니다. Flow cytometry, WB, IHC 등 다양한 응용에 적합하며, 0.5 mg/mL 농도의 액상 형태로 제공됩니다. 높은 순도와 안정적인 성능을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 3 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | - | View 4 publications |
| Flow Cytometry (Flow) | 1 µg/test | View 29 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2B11 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_842770 |
Available Conjugated Formats
- Alexa Fluor 488
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 805
- eFluor 450
- Functional Grade
- PE
- PE-Cyanine5.5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600
- Request custom conjugation available
Product Specific Information
The 2B11 monoclonal antibody reacts with mouse and human CXCR4 (CD184, Fusin, LESTR, HUMSTR). CXCR4 is a G-protein-coupled chemokine receptor that functions as a coreceptor for X4 HIV-1 entry into CD4+ cells and plays a key role in lymphocyte migration. Its ligand is SDF1, and it is essential for granulocyte progenitor maintenance and B cell development.
Expression of CXCR4 differs between species. In mice, CXCR4 is restricted to CD4+CD8+ thymocytes, while in humans it is predominantly expressed on B lymphocytes in the spleen.
The 2B11 antibody recognizes the amino terminus of CXCR4 and induces receptor internalization without blocking SDF1α binding.
Applications include flow cytometry, Western blotting, and frozen tissue immunohistology.
Tested Application: Flow cytometric analysis of mouse thymocytes (≤1 µg/test, 100 µL final volume).
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CXCR4 (Fusin, LESTR, HUMSTR) belongs to the G-protein-coupled chemokine receptor family with seven transmembrane domains. It acts as a co-receptor for HIV-1 and HIV-2 entry via CD4 binding and mediates Env-induced fusion. It also serves as a receptor for CXCL12/SDF-1, leading to calcium signaling and MAPK activation.
CXCR4 has roles in hematopoiesis, cardiac and vascular development, and neuronal survival. It binds bacterial LPS and mediates inflammatory responses. Antibodies against CXCR4 can block HIV-1 and HIV-2 fusion and infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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