Thermo Fisher Scientific CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), eBioscience

Thermo Fisher Scientific CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugated Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661
• eFluor 450
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation available

Product Specific Information

Description:
The HIR2 monoclonal antibody reacts with human glycophorin A, a sialoglycoprotein expressed by erythroid precursors and mature circulating red cells.

Applications Reported:
HIR2 can be used in flow cytometric analysis.

Applications Tested:
The HIR2 (GA-R2) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and cell agglutination assay. Recommended usage is approximately 0.1 µg per test (100 µL final volume). Optimal titration is advised depending on cell number (10⁵–10⁸ cells/test).

Purity: Greater than 90%, determined by SDS-PAGE.
Aggregation: Less than 10%, determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD235a (Glycophorin A, GPA) is a single-pass membrane sialoglycoprotein expressed in mature erythrocytes and erythroid precursor cells. It carries blood group M and N specificities and may act as a barrier to cell fusion, reducing red blood cell aggregation. CD235a also serves as a receptor for Sandei virus, parvovirus, and Streptococcus adhesin Hsa.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.