Thermo Fisher Scientific MDM2 Monoclonal Antibody (OTI3F1), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).

Target Information

MDM2 is a ubiquitin ligase for p53 and plays a central role in regulating p53 stability.
Located on chromosome 12q, MDM2 phosphorylation by Akt at Ser166 and Ser186 enhances interaction with p300, promoting p53 ubiquitination and degradation.
Phosphorylation also blocks binding to p19ARF, further increasing p53 degradation.
MDM2 negatively regulates p53 function by inhibiting its transactivation and promoting its degradation through E3 ubiquitin ligase activity.
Overexpression of MDM2 leads to inactivation of p53 and reduced tumor suppressor activity.
MDM2 is implicated in apoptosis and cell cycle regulation and is overexpressed in various malignancies such as soft tissue carcinomas and breast cancer.
Additionally, MDM2 interacts with proteins including retinoblastoma 1 and ribosomal protein L5.
More than 40 alternatively spliced transcript variants have been identified in tumor and normal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.