Thermo Fisher Scientific MDM2 Monoclonal Antibody (OTI22D6), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

MDM2 is a ubiquitin ligase for p53 and plays a central role in regulating p53 stability. It is located on chromosome 12q. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 enhances interaction with p300, promoting ubiquitination and degradation of p53.
MDM2 phosphorylation also inhibits binding to p19ARF, further increasing p53 degradation. MDM2 negatively regulates p53 function by inhibiting its transactivation activity and promoting its degradation via E3 ubiquitin ligase activity.
MDM2 is involved in apoptosis and cell cycle regulation and is overexpressed in various human malignancies such as soft tissue carcinomas and breast cancer. It interacts with proteins including retinoblastoma 1 and ribosomal protein L5. Over 40 alternatively spliced transcript variants have been identified in both tumor and normal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.