Thermo Fisher Scientific MDM2 Monoclonal Antibody (OTI20D1), TrueMAB

Applications

• Western Blot (WB): Tested dilution 1:500

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

MDM2 is a ubiquitin ligase for p53 and plays a central role in regulating p53 stability.
Located on chromosome 12q, MDM2 undergoes Akt-mediated phosphorylation at Ser166 and Ser186, enhancing its interaction with p300 and promoting p53 ubiquitination and degradation.
MDM2 negatively regulates p53 function by inhibiting its transactivation and facilitating its degradation through E3 ubiquitin ligase activity.
It is overexpressed in various human cancers, including soft tissue carcinomas and breast cancer.
Beyond p53 regulation, MDM2 influences apoptosis and cell cycle via interactions with proteins such as retinoblastoma 1 and ribosomal protein L5.
More than 40 alternatively spliced transcript variants of MDM2 have been identified in both tumor and normal tissues.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.