Thermo Fisher Scientific Gata-3 Monoclonal Antibody (TWAJ), Brilliant Violet 480, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular Staining)
• Tested Dilution: 5 µL (1.0 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• Brilliant UV 395, 563, 661
• Brilliant Violet 421, 605, 711, 786
• eFluor 450, 660
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a member of the Gata family of transcription factors.
Gata-3 is a T cell–specific transcription factor essential for thymic development and Th2 differentiation. It is expressed in the central nervous system, skin, mammary glands, and kidney during embryogenesis. Homozygous knockout of Gata-3 is embryonic lethal, highlighting its essential role in development.

In the thymus, expression is mainly found in CD4 single-positive cells. During Th2 differentiation, Gata-3 binds to the IL-4 promoter and represses T-bet expression, inhibiting Th1 differentiation.
Alternative splice variants (50 and 45 kDa) have been reported, and this antibody recognizes both forms.

• Buffer Requirement: Use Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00) for staining.
• Cross-reactivity: Published in rhesus monkey.
• Applications Reported: Intracellular staining followed by flow cytometric analysis.
• Applications Tested: Pre-diluted and validated for intracellular staining of mouse thymocytes using Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
  Recommended usage: 5 µL (1.0 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test).

Fluorochrome Information:
Brilliant Violet™ 480 (BV480) emits at 479 nm and is suitable for cytometers with a violet (405 nm) laser. Ensure instrument compatibility.

Staining Buffer Recommendation:
When using multiple polymer dye–conjugated antibodies (e.g., Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product #SB-4401-42) or Brilliant Stain Buffer™ (Product #00-4409-75) to reduce non-specific interactions.

Fixation Compatibility:
BV480 is not compatible with methanol-based fixation.

• Excitation: 440 nm
• Emission: 479 nm
• Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

The GATA3 gene encodes a zinc finger transcription factor that binds enhancer elements of all four T-cell receptor (TCR) genes (alpha, beta, gamma, delta).
It is abundantly expressed in T lymphocytes and plays a key role in T-cell receptor gene activation.
The human gene contains six exons over 17 kb of DNA, with zinc fingers encoded by two conserved exons similar to GATA1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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