Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), Brilliant Violet 480, eBioscience
마우스 Ly-6G(Gr-1) 항원을 인식하는 단일클론 항체로, 중성구 표지 및 분석에 적합. Brilliant Violet 480 형광체 결합으로 405 nm 레이저 기반 유세포분석에 최적. 4°C 암소 보관, 메탄올 고정 비호환. 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 1.0 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Brilliant Violet™ 480, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1A8-Ly6g |
| Conjugate | Brilliant Violet™ 480 |
| Excitation/Emission Max | 437 / 478 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925663 |
Additional Available Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 661 / 737
- Brilliant Violet 421 / 650 / 786
- eFluor 450
- FITC
- Functional Grade
- PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436 / 600 / 702
- Request custom conjugation
Product Specific Information
The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G (Gr-1), a 25 kDa GPI protein expressed exclusively by neutrophils. Ly-6G is associated with neutrophil infiltration, recruitment, and migration, and interacts with B2 integrins (CD11a, CD11b). The ligand for Ly-6G remains unknown.
Unlike the RB6-8C5 clone, 1A8-Ly6g binds specifically to Ly-6G on neutrophils and can be used for neutrophil depletion with reported antagonist activity.
Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse bone marrow cells at ≤1.0 µg/test.
A test is defined as the amount of antibody required to stain a cell sample in 100 µL final volume (10⁵–10⁸ cells/test). Titrate for optimal performance.
Fluorochrome Information:
Brilliant Violet™ 480 (BV480) emits at 479 nm and is suitable for cytometers with a 405 nm violet laser. Ensure instrument compatibility.
When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant UV™), use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.
Note: BV480 is not compatible with methanol-based fixation.
Excitation: 440 nm Emission: 479 nm Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
Ly6G (Lymphocyte antigen 6 complex locus G6D) is part of the myeloid differentiation antigen Gr-1 complex (with Ly6C).
It is a 21–25 kDa protein expressed by myeloid-derived cells in bone marrow and serves as a marker for peripheral neutrophils, monocytes, and granulocytes.
Expression correlates with granulocyte differentiation and maturation.
Ly6G is implicated in anti-tumor responses and may modulate local immune activity through neutrophil signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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