Thermo Fisher Scientific TCR beta Monoclonal Antibody (H57-597), Functional Grade, eBioscience

Thermo Fisher Scientific TCR beta Monoclonal Antibody (H57-597), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• eFluor 450
• eFluor 506
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 530
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 725
• NovaFluor Blue 800
• NovaFluor UV 765
• NovaFluor Violet 690
• NovaFluor Violet 800
• NovaFluor Yellow 810
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR. TCR beta is expressed by thymocytes in a developmentally regulated manner and by a majority of peripheral T cells. Crosslinking of the TCR complex with H57-597 induces activation and proliferation of T cells or apoptosis depending on assay conditions. It is used as a phenotypic marker for TCR beta–expressing T cells.

Applications Reported:

• Flow cytometric analysis
• Cross-linking of the T cell receptor for activation
• In vitro depletion assays

Applications Tested:

• Tested by flow cytometric analysis of mouse thymocytes and splenocytes
• Recommended ≤ 0.5 µg/test (100 µL volume, 10⁵–10⁸ cells/test)
• Optimize antibody concentration for each assay

Storage and Handling:

• Use in sterile environment
• 0.2 µm post-manufacturing filtration

Purity and Quality:

• Purity >90% (SDS-PAGE)
• Endotoxin <0.001 ng/µg (LAL assay)
• Aggregation <10% (HPLC)

Target Information

T cell receptors (TCRs) discriminate foreign from self-peptides presented by MHC class II molecules, critical for adaptive immunity. Recognition of self-peptides is linked to autoimmune diseases, while mutant self-peptides are associated with tumors. Superantigen engagement of TCRs can cause toxic shock syndrome. Autoantibodies to V beta segments are found in RA and SLE patients, potentially modulating inflammatory responses. Most human T cells express TCR alpha-beta with CD4 or CD8; gamma-delta T cells, found in epithelial tissues, exhibit distinct antigen recognition and immunoregulatory functions.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.