Thermo Fisher Scientific CD270 (HVEM) Monoclonal Antibody (LH1), Functional Grade, eBioscience
마우스 HVEM(TNFRSF14)을 인식하는 Armenian hamster 유래 단클론 항체입니다. Flow cytometry 및 기능 분석용으로 적합하며, 0.25 µg/test 농도로 사용됩니다. 1 mg/mL 액상형으로 제공되며, 4°C에서 보관합니다. Endotoxin 수준이 매우 낮고, 0.2 µm 여과 처리되었습니다.
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4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD270 (HVEM) Monoclonal Antibody (LH1), Functional Grade, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 2 publications |
| Functional Assay | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | LH1 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
| RRID | AB_2866146 |
Additional Formats
Product Specific Information
The LH1 monoclonal antibody reacts with mouse Herpes Virus Entry Mediator (HVEM, TR2), a member of the TNF-receptor superfamily. HVEM is expressed on various cell types, including T cells, B cells, monocytes, neutrophils, and dendritic cells. It functions as a cellular mediator for herpes simplex virus (HSV) entry via binding with viral envelope glycoprotein D (gD). The cytoplasmic region of HVEM interacts with TRAF family members, mediating immune response signaling pathways.
HVEM also serves as a ligand for BTLA (B and T lymphocyte attenuator), indicating its role in lymphocyte activation and immune homeostasis. The LH1 antibody has been reported as a blocking antibody that interferes with the HVEM-LIGHT interaction but not with HVEM-BTLA binding.
Applications Reported: Functional assays and flow cytometric analysis (fluorochrome-conjugated LH1 recommended for flow cytometry).
Applications Tested: Validated for flow cytometric analysis of mouse splenocytes. Recommended usage ≤0.25 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Optimal titration is advised for specific assays.
Storage and Handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).
Target Information
Gene: TNFRSF14 (HVEM)
Function: Member of the TNF-receptor superfamily; mediates HSV entry via glycoprotein D binding. The cytoplasmic region binds TRAF family members, activating immune signaling pathways.
Biological Role: Activation of TNFRSF14 in T cells stimulates proliferation and cytokine production, enhancing CTL-mediated tumor immunity. Multiple alternatively spliced transcript variants have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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