Thermo Fisher Scientific alpha-1 Antitrypsin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 1 publication 1 publication

ELISA (ELISA)

1:1,000-1:5,000

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Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

Mix of synthetic peptides corresponding to residues 30-41 and 296-307 of human alpha-1 antitrypsin protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

IgY fraction

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2185300

Product Specific Information

PA1-9529 detects Alpha1 trypsin in human samples.

PA1-9529 has been successfully used in Western blot and ELISA procedures. By Western blot, PA1-9529 detects a 46 kDa protein representing Alpha1 trypsin.

PA1-9529 immunogen is a synthetic peptide based on the human alpha-1 antitrypsin protein.

Target Information

Alpha-1-AT is synthesized in the liver and it acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leucocytic proteases, plasmin, and thrombin, which may be released during inflammatory reactions in the lung. In the absence of alpha-1-AT, these enzymes are not inhibited and they may digest pulmonary parenchyma. Alpha-1-AT deficiency is associated with chronic obstructive lung disease (emphysema) and less frequently with hepatic cirrhosis in infants and respiratory distress of the newborn. Increase in alpha-1-AT occurs as an acute phase response to tissue necrosis and inflammation. Serum level of alpha-1-AT is elevated in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.