Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), NovaFluor Blue 660-40S, eBioscience

Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), NovaFluor Blue 660-40S, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: ≤ 0.4 µg/test

Product Specifications

Product Specific Information

The M1/70 monoclonal antibody reacts with mouse CD11b, a 165–170 kDa integrin alphaM. CD11b associates with CD18 to form the alphaMbeta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen.
Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes, and mouse B-1 cells in the peritoneal cavity.
M1/70 is also cross-reactive to human CD11b, allowing detection on human peripheral blood monocytes, granulocytes, and NK cell subsets.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes and bone marrow cells (≤ 0.4 µg per test; 10⁵–10⁸ cells/test)

Recommendations:

• Titrate antibody for optimal performance
• Avoid DNA intercalating viability dyes (PI, 7-AAD, DAPI)
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for NovaFluor dyes
• Add 5 µL of CellBlox Plus Blocking Buffer per sample (Cat. No. C001T06F01)

NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures engineered for optimized fluorescence and minimal spillover.

Excitation: 509 nm
Emission: 665 nm
Laser: 488 nm (Blue)

Target Information

CD11b (integrin alpha-M, ITGAM) forms a heterodimer with CD18 (integrin beta-2), known as Mac-1 or CR3.
It is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, subsets of NK and dendritic cells, and microglia.
Acts as a receptor for ICAMs, iC3b, fibrinogen, and Factor X, facilitating cell-cell and cell-matrix interactions.
Commonly used as a microglial marker; expression increases during monocyte maturation.
Associated with systemic lupus erythematosus and ITGAM-related autoimmune susceptibility.

For Research Use Only. Not for use in diagnostic procedures.

Additional Formats Available

• Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin
• Brilliant UV / Brilliant Violet series, eFluor dyes, FITC, PE, PE-Cyanine, PerCP conjugates
• Super Bright series and NovaFluor Blue/Yellow variants
• Custom conjugation available upon request

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