Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), NovaFluor Red 700, eBioscience

Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), NovaFluor Red 700, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.4 µg/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV / Violet series (395–805 nm)
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue / Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright series (436–780 nm)
• Custom conjugation available

Product Specific Information

The M1/70 monoclonal antibody reacts with mouse CD11b, a 165–170 kDa integrin alphaM that forms a non-covalent complex with CD18 (Mac-1, αMβ2 integrin). It binds to CD54 (ICAM-1), C3bi, and fibrinogen, and is expressed by macrophages, NK cells, granulocytes, activated lymphocytes, and mouse B-1 cells.
M1/70 also cross-reacts with human CD11b, enabling detection on human peripheral blood monocytes, granulocytes, and NK cell subsets.

Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometry of mouse splenocytes and bone marrow cells, ≤0.4 µg/test (100 µL final volume). Optimal titration recommended.

Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer, recommended for all NovaFluor staining procedures.

• Add 5 µL per sample (10³–10⁸ cells) for single-color or cocktail staining.
• Excitation: 639 nm; Emission: 700 nm; Laser: 633–640 nm (Red).

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures optimized for minimal spillover and spread.

Target Information

CD11b (integrin alpha-M, ITGAM) is a 165 kDa adhesion molecule forming a heterodimer with CD18 (integrin beta-2). Known as Mac-1 or CR3, CD11b/CD18 acts as a receptor for ICAMs, CD14, CD23, fibrinogen, and Factor X.
Expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and microglia.
CD11b expression varies by tissue and increases during monocyte maturation. It is a key marker for microglial identification and cell adhesion studies.
Associated with systemic lupus erythematosus and ITGAM-related autoimmune susceptibility.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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