Thermo Fisher Scientific Phospho-PDPK1 (Tyr9) Polyclonal Antibody

Thermo Fisher Scientific Phospho-PDPK1 (Tyr9) Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose, then affinity purified using phospho-PDK1 (Tyr-9) peptide.
The purified antibody detects a 65 kDa protein corresponding to the apparent molecular mass of PDK1 on SDS-PAGE immunoblots of human A431 cells treated with EGF, IGF-1, or pervanadate, but not in untreated cells.
PDK1 (Tyr-9) reactivity was also observed in adult mouse brain.

Target Information

PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases.
PDPK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop.
It also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate.
Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), leading to the dephosphorylation and activation of glycogen synthase and stimulation of glycogen synthesis.
Because of the role that PDPK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.