Thermo Fisher Scientific Phospho-PLCG1 (Tyr775) Polyclonal Antibody
PLCγ1 (Tyr775) 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal Antibody. Western blot, ICC/IF, IP에 사용 가능. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제된 고품질 항체로 연구용에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | 1:100 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PLCγ1 (Tyr-775) synthetic peptide (coupled to carrier protein) corresponding to amino acids around tyrosine 775 in human PLCγ1. This sequence has high homology to the conserved site in rat and mouse PLCγ1, and low homology to PLCγ2. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942972 |
Product Specific Information
This antibody was cross-adsorbed to a non-specific phospho-tyrosine peptide then affinity-purified using phospho-PLCγ1 (Tyr-775) peptide.
The antibody detects a 150 kDa protein in human Jurkat and A431 cells treated with pervanadate, but is not observed in untreated cells.
Target Information
The mammalian Phospholipase Cγ (PLCγ) family includes two closely related proteins, PLCγ1 and PLCγ2.
PLCγ isozymes contain core structural domains and a unique array of domains including an additional PH domain, two SH2 domains, and one SH3 domain.
In response to extracellular stimuli such as hormones and growth factors, receptor tyrosine kinases (RTKs) phosphorylate and activate PLCγ.
Activated PLCγ catalyzes hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to produce the metabolic second messenger molecules inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG).
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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