Thermo Fisher Scientific Phospho-Paxillin (Ser83) Polyclonal Antibody
Phospho-Paxillin (Ser83) 폴리클로날 항체로, 마우스 반응성 및 Rabbit IgG 호스트를 사용합니다. Western blot과 Immunocytochemistry에 적합하며, 액상 형태로 항원 친화 크로마토그래피로 정제되었습니다. -20°C에서 보관하며 연구용으로만 사용됩니다.
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Thermo Fisher Scientific Phospho-Paxillin (Ser83) Polyclonal Antibody
Applications and Tested Dilution
- Western Blot (WB): 1:1,000
- Immunocytochemistry (ICC/IF): 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-Paxillin (Ser-83) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 83 of mouse paxillin. This sequence is highly conserved in rat and human paxillin and is also found in all isoforms (a, b, g) of paxillin. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2942964 |
Product Specific Information
This antibody detects a 68 kDa protein corresponding to phosphorylated paxillin on SDS-PAGE immunoblots of NGF-treated rat PC12 cells, as well as calyculin A-treated human A431 cells and mouse macrophages.
Target Information
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. Its tyrosine phosphorylation is influenced by cell-cell adhesion changes. Paxillin interacts tightly with FAK and Crk through its SH2 domain, independent of the extracellular matrix. Although initially identified in fibroblasts, paxillin phosphorylation may also play a role in neurite extension during differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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