Thermo Fisher Scientific NGF Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Reconstitute in 100 µL of sterile ultrapure water. Centrifuge to remove any insoluble material.
Store lyophilized antibody at 2–8 °C. After reconstitution, keep aliquots at −20 °C to −80 °C for higher stability, and at 4 °C with an appropriate antibacterial agent.
Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for additional stability.

Target Information

Nerve Growth Factor (NGF) influences the survival and differentiation of various neuronal and non-neuronal cells. NGF binds to specific cell surface receptors, existing as high and low affinity forms. Only the high affinity receptor mediates signal transduction.
Cross-linking studies with ¹²⁵I-NGF show that the high affinity receptor of rat PC12 cells forms a 158 kDa complex with NGF, while the low affinity receptor forms a 100 kDa complex.
Full-length cDNA clones for both human and rat NGF receptor genes exhibit over 90% homology. NGF promotes nerve growth and differentiation of sympathetic and sensory neurons.
Mutations in the NGF gene are associated with hereditary sensory and autonomic neuropathy type 5 (HSAN5), and dysregulation of NGF expression is linked to allergic rhinitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.