Thermo Fisher Scientific NGF Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Reconstitute in 500 µL of sterile, ultrapure water.
• Centrifuge to remove any insoluble material.
• Store lyophilized antibody at 2–8°C.
• After reconstitution, store aliquots at -20°C to -80°C for higher stability, or at 4°C with antibacterial agent.
• Avoid repetitive freeze/thaw cycles.
• Glycerol (1:1) may be added for additional stability.

Target Information

Nerve growth factor (NGF) influences the survival and differentiation of various neuronal and non-neuronal cells. NGF binds to specific high- and low-affinity cell surface receptors, with only the high-affinity receptor capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high-affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF, while the low-affinity receptor forms an Mr 100,000 complex.
Full-length cDNA clones for both human and rat NGF receptor genes show greater than 90% homology. NGF exhibits nerve growth stimulating activity and regulates the growth and differentiation of sympathetic and sensory neurons.
Mutations in the NGF gene are associated with hereditary sensory and autonomic neuropathy type 5 (HSAN5), and dysregulation of NGF expression is linked to allergic rhinitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.