Thermo Fisher Scientific CHEK2 Monoclonal Antibody (OTI2H5), TrueMAB
CHEK2 단백질을 표적으로 하는 단클론 항체로, Western blot, IHC, Flow cytometry, IP 등 다양한 응용에 적합. 인간 및 개 반응성. 동결건조 형태로 높은 순도(친화 크로마토그래피 정제) 보유. 연구용으로 DNA 손상 반응 및 암 관련 연구에 활용 가능.
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Thermo Fisher Scientific CHEK2 Monoclonal Antibody (OTI2H5), TrueMAB
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
| Immunoprecipitation (IP) | 2 µg/500 µL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human |
| Host/Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2H5 |
| Immunogen | Full length human recombinant protein of human CHEK2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
CHEK2 (CHK2) is a serine/threonine kinase involved in the DNA damage checkpoint pathway. Mutations in CHK2 are associated with various cancers. Upon DNA damage, CHK2 phosphorylates several cell cycle regulators, including tumor suppressor proteins such as BRCA1, aiding in cellular survival.
CHK2 acts as a tumor suppressor, with mutations linked to Li-Fraumeni syndrome and predisposition to sarcomas, breast cancer, and brain tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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