Thermo Fisher Scientific CHEK2 Monoclonal Antibody (OTI4C10), TrueMAB
CHEK2 단백질을 인식하는 Mouse 단일클론 항체로, Western blot, IHC, Flow cytometry, IP 등에 사용 가능. 인간 및 개 반응성. 동결건조 형태로 제공되며, 높은 순도(affinity chromatography 정제)와 안정적 저장(-20°C) 조건을 가짐.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
| Immunoprecipitation (IP) | 2 µg/500 µL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4C10 |
| Immunogen | Full length human recombinant CHEK2 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
CHEK2 (CHK2) is a serine/threonine kinase involved in the DNA damage checkpoint pathway. Mutations in CHK2 are associated with various cancers, including Li-Fraumeni syndrome, sarcomas, breast cancer, and brain tumors.
CHK2 phosphorylates tumor suppressor proteins such as BRCA1, aiding in cell survival after DNA damage. It functions as a tumor suppressor, with mutations linked to cancer predisposition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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