Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (429), eBioscience

Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (Clone 429)

Applications and Tested Dilutions

Product Specifications

Available Conjugated Formats

• APC (Cat. No. 17-1061-82)
• Biotin (Cat. No. 13-1061-82)
• eFluor 450 (Cat. No. 48-1061-82)
• eFluor 660 (Cat. No. 50-1061-82)
• FITC (Cat. No. 11-1061-82)
• Functional Grade (Cat. No. 16-1061-82)
• PE (Cat. No. 12-1061-82)
• PE-Cyanine7 (Cat. No. 25-1061-82)
• Custom conjugation available upon request

Product Specific Information

The 429 monoclonal antibody reacts with mouse CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells express low levels of CD106 constitutively and upregulate it upon cytokine stimulation. CD106 binds to integrin α4β1 (VLA-4, CD49d/CD29) and integrin α4β7 (LPAM-1), which are important for lymphocyte and myeloid development in bone marrow and thymus. Cytokine-mediated upregulation of CD106 suggests a role in inflammatory responses.

Applications Reported:

• Flow cytometric analysis
• Immunoprecipitation
• Immunohistochemical staining (IHC-F)
• Functional blocking assays (use Functional Grade purified 429 antibody, Product #16-1061-82)

Applications Tested:
Tested by flow cytometric analysis of mouse bone marrow cells. Recommended ≤0.25 µg per test (100 µL final volume). Optimize antibody titration for best performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. It regulates leukocyte migration and adhesion, plays a role in signal transduction, and is implicated in inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.