Thermo Fisher Scientific CD107b (LAMP-2) Monoclonal Antibody (eBioABL-93 (ABL-93)), eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugated Formats

• Alexa Fluor 488
• eFluor 660
• FITC
• Request custom conjugation

Product Specific Information

The eBioABL-93 monoclonal antibody reacts with mouse CD107b (LAMP-2), a highly glycosylated lysosomal membrane protein (~120 kDa). It is expressed intracellularly and transiently on cytolytic T cell surfaces during degranulation. CD107b may be identical to the mouse Mac-3 antigen.

Applications Reported:

• Intracellular staining followed by flow cytometric analysis
• Immunoprecipitation
• Immunohistochemical staining
• Surface staining in flow cytometry-based degranulation assays

Applications Tested:
Tested by intracellular staining and flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. Recommended ≤0.5 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Antibody titration is advised for optimal assay performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

LAMP2 (CD107b) is a lysosome-associated membrane glycoprotein involved in lysosome biogenesis and fusion with phagosomes. It plays roles in:

• Tumor cell metastasis
• Lysosomal maintenance and adhesion
• Chaperone-mediated autophagy

Structure:
Large amino-terminal intra-lysosomal domain, hydrophobic transmembrane domain, and short cytoplasmic tail.
Molecular Weight: ~100–130 kDa (mature glycosylated form)
Variants: LAMP-2A, LAMP-2B, LAMP-2C

LAMP2 deficiency leads to accumulation of autophagosomes, causing cardiomyopathy and myopathy (Danon’s disease). Together with LAMP-1 (CD107a), it is a major lysosomal membrane component.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.