Thermo Fisher Scientific CD104 (Integrin beta 4) Monoclonal Antibody (439-9B), eBioscience
Thermo Fisher Scientific CD104 (Integrin beta 4) Monoclonal Antibody (Clone 439-9B)은 인간 CD104 단백질을 인식하는 Rat IgG2b 항체로, Flow Cytometry, IHC, WB 등 다양한 분석에 사용됩니다. 높은 순도(>90%)와 안정성으로 세포 부착 및 종양 관련 연구에 적합합니다.
- 카탈로그번호
- 14-1049-xx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD104 (Integrin beta 4) Monoclonal Antibody (439-9B), eBioscience
Thermo Fisher Scientific CD104 (Integrin beta 4) Monoclonal Antibody (439-9B), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-Dependent |
| Immunohistochemistry (IHC) | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| Flow Cytometry (Flow) | 1 µg/test |
| Immunoprecipitation (IP) | Assay-Dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rat / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 439-9B |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2864964 |
Additional Formats
Product Specific Information
The monoclonal antibody 439-9B recognizes human CD104 (Integrin beta 4), a 202 kDa subunit forming a laminin receptor with integrin alpha 6 (CD49f). CD104 is expressed on epithelial and tumor cells, involved in cell-matrix and cell-cell interactions, and plays a role in tumorigenicity. This antibody cross-reacts with bovine and does not affect adhesion of human colon adenocarcinoma cells.
Applications Reported:
Suitable for flow cytometry, immunoprecipitation, immunoblotting (nonreducing conditions), and immunohistology of frozen and paraffin-embedded sections.
Applications Tested:
Validated in flow cytometric analysis of A549 cells at ≤1 µg/test (100 µL volume). Cell count range: 10⁵–10⁸ cells/test. Antibody titration is recommended for optimal assay performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Integrins are heterodimeric transmembrane glycoprotein receptors composed of alpha and beta subunits. They mediate cell-matrix and cell-cell adhesion and regulate gene expression and cell growth. The integrin beta 4 subunit forms a receptor for laminins, often associating with alpha 6 subunit, and plays a critical role in invasive carcinoma biology. Mutations in this gene are linked to epidermolysis bullosa with pyloric atresia. Multiple splice variants exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.