Thermo Fisher Scientific KDEL Polyclonal Antibody
KDEL 서열을 인식하는 Rabbit Polyclonal Antibody로, GRP78·GRP94·PDI 등 ER 단백질 검출에 적합합니다. WB, IHC, IF, IP 등 다양한 응용 가능하며, 항원 친화 크로마토그래피로 정제된 고품질 제품입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 8–16 µg/mL | 4 publications |
| Immunohistochemistry (IHC) | – | 6 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:200 | – |
| Immunocytochemistry (ICC/IF) | Assay-dependent | 11 publications |
| Flow Cytometry (Flow) | 1–20 µg/mL | – |
| Immunoprecipitation (IP) | Assay-dependent | 1 publication |
| Miscellaneous PubMed (Misc) | – | 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Hamster, Human, Mouse, Rat |
| Published Species | Hamster, Human, Mouse, Rat, Sea urchin |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_325593 |
Product Specific Information
PA1-013 detects KDEL from human, rat, mouse, and hamster.
This antibody has been successfully used in Western blot, immunofluorescence, and immunoprecipitation procedures.
- Western Blot Results: Detects a 57 kDa protein (PDI), a 78 kDa protein (GRP78), and an ~94 kDa protein (GRP94) in mouse myeloma cells.
- Immunofluorescence: Staining of GRP78 in mouse myeloma cells shows diffuse cytoplasmic staining consistent with ER localization.
- Immunoprecipitation: PA1-013 immunoprecipitates PDI, GRP78, an ~72 kDa ERp72 protein, and GRP94 from rodent tissues.
Reconstitution: Reconstitute in 100 µL PBS to create a stock of 1 mg/mL.
Target Information
The sequence Lys-Asp-Glu-Leu (KDEL) or closely related motifs are found at the C-terminus of soluble ER-resident and some membrane proteins.
GRP78 (78 kDa), GRP94 (94 kDa), and PDI share this C-terminal KDEL sequence.
This motif is essential for ER retention and reduces secretion of proteins from the ER, mediated by the KDEL receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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