Thermo Fisher Scientific Phospho-CRYAB (Ser59) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

8 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Bovine, Human, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues F(54) L R A P S(p) W I D T G(64) of human alpha-B Crystallin. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CRYAB,uniProtId:P02511-1,ncbiNodeId:9606,antigenRange:54-64,antigenLength:175,antigenImageFileName:PA1-012_CRYAB_P02511-1_Rabbit.svg,antigenImageFileNamePDP:PA1-012_CRYAB_P02511-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 30mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2245283

Product Specific Information

PA1-012 detects phospho-alpha-B crystallin (Ser59) from bovine, human and rat samples. This antibody does not detect the unphosphorylated form of the protein.

PA1-012 has been successfully used in Western blot, immunohistochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~20 kDa protein representing recombinant bovine phospho-alpha-B crystallin (Ser59). Immunofluorescence staining of phospho-alpha-B crystallin (Ser59) in human brain from Alexander`s diseased patients results in staining of cells in the cerebral cortex.

The PA1-012 immunogen is a synthetic phosphopeptide corresponding to residues F(54) L R A P S(p) W I D T G(64) of human alpha-B Crystallin.

Reconstitute in 100 µL PBS to create a stock of 1 mg/mL.

Target Information

Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products-alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander`s disease where it accumulates in brain cells of those afflicted.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.