Thermo Fisher Scientific KDEL Polyclonal Antibody, Alexa Fluor 488

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1-10 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:KDEL,uniProtId:Q66HD0-1,ncbiNodeId:10114,antigenRange:643-654,antigenLength:804,antigenImageFileName:PA1-013-A488_KDEL_Q66HD0-1_Rabbit.svg,antigenImageFileNamePDP:PA1-013-A488_KDEL_Q66HD0-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2662647

Product Specific Information

PA1013A488 detects KDEL from human, rat, mouse and hamster.

The PA1013A488 immunogen is a synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94.

Target Information

The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins, GRP78 and GRP94 respectively, and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.