Thermo Fisher Scientific KDEL Polyclonal Antibody, Alexa Fluor 488
Alexa Fluor 488으로 표지된 KDEL 폴리클로날 항체로, 인간·쥐·햄스터·마우스 시료에 반응합니다. ER 단백질의 KDEL 서열을 인식하며, WB, IHC, ICC/IF에 사용 가능합니다. 항원 친화 크로마토그래피로 정제된 고순도 항체입니다.
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Applications
Western Blot (WB)
- Tested Dilution: Not specified
- Publications: View 2 publications
Immunohistochemistry (IHC)
- Tested Dilution: Not specified
- Publications: View 1 publication
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1–10 µg/mL
- Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Hamster, Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2662647 |
Product Specific Information
PA1-013-A488 detects KDEL from human, rat, mouse, and hamster.
The immunogen is a synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94.
Target Information
The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins.
78 and 94 kDa glucose-regulated proteins (GRP78 and GRP94) and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence.
This sequence is essential for ER retention and sufficient to reduce secretion of proteins from the ER.
Retention is mediated by a KDEL receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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