Thermo Fisher Scientific KDEL Polyclonal Antibody, Alexa Fluor 647
Alexa Fluor 647로 표지된 KDEL 폴리클로날 항체로, 인간·쥐·생쥐·햄스터 시료에서 ER 단백질 KDEL 서열 검출에 사용됩니다. WB, IHC, ICC/IF에 적합하며 항원 친화 크로마토그래피로 정제되었습니다. 4°C 암소 보관, 연구용 전용.
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Thermo Fisher Scientific KDEL Polyclonal Antibody, Alexa Fluor 647
Applications and Tested Dilution
- Western Blot (WB): Not specified
- Immunohistochemistry (IHC): Not specified
- Immunocytochemistry (ICC/IF): 1–10 µg/mL
Publications
- WB: View 2 publications
- IHC: View 1 publication
- ICC/IF: View publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Hamster, Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94 |
| Conjugate | Alexa Fluor™ 647 |
| Excitation / Emission Max | 650 / 671 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2662417 |
Product Specific Information
PA1013A647 detects KDEL from human, rat, mouse and hamster.
The immunogen is a synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94.
Target Information
The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins.
GRP78 (78 kDa) and GRP94 (94 kDa), as well as protein disulfide isomerase (PDI), share the C-terminal KDEL sequence.
This motif is essential for ER retention and sufficient to reduce protein secretion from the ER, mediated by the KDEL receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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